Detecting toxicological responses in cells with the bioluminescent GSH-Glo(TM) Glutathione Assay
نویسندگان
چکیده
Glutathione (GSH), a tripeptide (L γ-glutamyl-cysteinyl-glycine) is the major nonprotein thiol found abundantly in eukaryotic cells (1–3). GSH plays a critical role in many metabolic pathways. It acts as a cofactor for intracellular enzymes like the glutathione S-transferases (GST) and glutathione peroxidase; it aids in amino acid transport and is essential for detoxification of xenobiotics and reactive oxygen species (ROS). GSH is also involved in maintenance of cellular redox-regulated signal transduction. Reactive oxygen species and unintended drugdrug interactions often cause a drop in GSH levels by reaction DETECTING TOXICOLOGICAL RESPONSES IN CELLS WITH THE BIOLUMINESCENT GSH-GLOTM GLUTATHIONE ASSAY
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